Microperfusion Pump

Product Information Microperfusion Pump

04-20 Microperfusion pump with pump spindle, motor drive, and control unit
04-21 Microperfusion pump with pump spindle
04-22 Motor drive for microperfusion pump inserted
04-23 Control unit for microperfusion pump

Perfusion pump (04-21)

Motor drive (04-22) with perfusion pump

Control unit (04-23)

Description:

Microperfusion pump 0-40 nl/min or 0-80 nl/min. Available complete with motor drive and control unit (04-20), or separate as pump only (04-21), motor drive only (04-22), and control unit only (04-23). Works with perfusion capillaries of <1.0 mm outer diameter and >0.5 mm inner diameter.

cross section through perfusion pump

a)	end cap (front)	b)	brass bushing	c)	silicone tubing	d)	compression screw
e)	Teflon sealing cone	E)	venting hole	f)	pump spindle housing	g)	piston
h)	pump spindle	i)	drive shaft housing	k)	locking pin	l)	drive shaft
m)	end cap (back)	n)	Teflon gaskets	o)	motor coupling	p)	motor drive

Mounting of Perfusion Capillary:

Remove pieces a-d shown in the figure. Do not remove Teflon sealing cone (e).
Hold pump upright (front up) and fill chamber R with mineral oil. Remove all air bubbles.
Replace compression screw (d) and tighten it securely by hand. Insert silicone tubing (c). Adjust the pump spindle so that the piston protrudes the front end of the compression screw by about 2 mm.
Funnel perfusion capillary through end cap (a) and brass bushing (b). Capillary should be completely filled with fluid.
Carefully slide the perfusion capillary over the piston until the back of the capillary touches the Teflon cone. Note that the capillary has to pass through the silicone tubing (c).
Push the brass bushing into the recess of the compression screw and replace end cap. While firmly tightening the end cap, push the capillary down to prevent it from slipping back.

Hints for Trouble-free Operation:

The system has to be absolutely free of air bubbles to allow backlash-free pumping. The back of the perfusion capillary must be made burr-free by fire-polishing to prevent scratching of Teflon sealing cone and silicone tubing. Also, avoid scratches on the pump piston and on the sealing surface of the compression screw. Remove Teflon cone only with supplied special tool.

Keep ambient temperature constant as thermal expansion or shrinkage of capillary and pump piston may lead to significant errors in the perfusion rate. At end of the experiment, release pressure on Teflon seal and silicone tubing by loosening front end cap and compression screw and remove perfusion capillary.

Accessories:

Spare spindle (04-25). Bushing and silicon tubing on request. See price list for other accessories.

©Vestavia Scientific, LLC [1/1/2008]